Journal article
A. Naik, Brent J. Formosa, Rishika G. Pulvender, Asiri G. Liyanaarachchi, B. Jena
bioRxiv, 2019
bioRxiv, 2019
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APA
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Naik, A., Formosa, B. J., Pulvender, R. G., Liyanaarachchi, A. G., & Jena, B. (2019). vH+-ATPase-induced intracellular acidification is critical to glucose-stimulated insulin secretion. BioRxiv.
Chicago/Turabian
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Naik, A., Brent J. Formosa, Rishika G. Pulvender, Asiri G. Liyanaarachchi, and B. Jena. “VH+-ATPase-Induced Intracellular Acidification Is Critical to Glucose-Stimulated Insulin Secretion.” bioRxiv (2019).
MLA
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Naik, A., et al. “VH+-ATPase-Induced Intracellular Acidification Is Critical to Glucose-Stimulated Insulin Secretion.” BioRxiv, 2019.
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@article{a2019a,
title = {vH+-ATPase-induced intracellular acidification is critical to glucose-stimulated insulin secretion},
year = {2019},
journal = {bioRxiv},
author = {Naik, A. and Formosa, Brent J. and Pulvender, Rishika G. and Liyanaarachchi, Asiri G. and Jena, B.}
}
Abstract
Swelling of secretory vesicles is critical for the regulated expulsion of intra-vesicular contents from cells during secretion. At the secretory vesicle membrane of the exocrine pancreas and neurons, GTP-binding G proteins, vH+-ATPase, potassium channels and AQP water channels, are among the players implicated in vesicle volume regulation. Here we report in insulin secreting MIN6 cells, the requirement of vH+-ATPase-mediated intracellular acidification, on glucose-stimulated insulin release. MIN6 cells exposed to the vH+-ATPase inhibitor Bafilomycin A show decreased acidification of the cytosolic compartment that include insulin-carrying granules. Additionally, a loss of insulin granule association with the cell plasma membrane is demonstrated and results in a decrease in glucose-stimulated insulin secretion and accumulation of intracellular insulin. These results suggest that vH+-ATPase-mediated intracellular acidification is required both at the level of secretory vesicles and the cell plasma membrane for cell secretion.
